Molecular Systems Biology

Complex‐centric proteome profiling by SEC‐SWATH‐MS

The study presents an integrated framework for targeted, complex‐centric analysis based on size exclusion chromatography (SEC) and SWATH/DIA mass spectrometry. The workflow facilitates the parallel detection of hundreds of protein complexes and their variants at high selectivity and under error‐control. Moritz Heusel, Isabell Bludau, George Rosenberger, Robin Hafen, Max Frank, Amir Banaei‐Esfahani, Audrey van Drogen, Ben C Collins, Matthias Gstaiger and Ruedi Aebersold Published online 14.01.2019

Widespread inter‐individual gene expression variability inArabidopsis thaliana

RNA‐seq on individual seedlings is used to detect inter‐individual gene expression variability in Arabidopsis thaliana. 9% of the transcriptome is identified as highly variable for at least one time point during the day/night cycle. Sandra Cortijo, Zeynep Aydin, Sebastian Ahnert and James CW Locke Published online 24.01.2019