GSE103305 Thyroid State Regulates Gene Expression in Human Whole Blood Cells

Contributors : Elske T Massolt ; Mirjam C van den Hout-van Vroonhoven ; Selmar Leeuwenburgh ; Robin P Peeters ; Theo J Visser ; Marcel E Meima ; W.Edward E Visser
Series Type : Expression profiling by high throughput sequencing
Organism : Homo sapiens

Context: Despite the well-recognized clinical features due to insufficient or excessive thyroid hormone (TH) levels in humans, it is largely unknown which genes are regulated by TH in human tissues. objective: To study the effect of TH on human gene expression profiles in whole blood, mainly consisting of TRα-expressing cells. Methods: We performed next-generation RNA sequencing on whole blood samples from 8 athyroid patients (4 females) on and after 4 weeks off levothyroxine replacement. Gene expression changes were analyzed through paired differential expression analysis and confirmed in a validation cohort. Weighted gene co-expression network analysis (WGCNA) was applied to identify thyroid state-related networks. Results: We detected 486 differentially expressed (DE) genes (fold-change above 1.5; multiple testing corrected P-value

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