GSE97716 Lin37 is essential for repression of cell-cycle genes in quiescence

Contributors : Christina S Mages ; Axel Wintsche ; Stephan H Bernhart ; Gerd A Muller
Series Type : Expression profiling by high throughput sequencing
Organism : Mus musculus

The DREAM complex represses cell-cycle gene transcription in quiescence. To investigate the role of the DREAM component Lin37, we created Lin37 knockout cells by CRISPR/Cas9-nickase. pRTS episomal vectors expressing luciferase or Lin37 were introduced in two independent knockout clones (411-27 and 632-2) to create Lin37 knockout and rescue cells. To exit the cell cycle and to enter quiescence, cells were serum starved for 60h. RNA-seq was applied to identify genes de-regulated by loss of Lin37 during quiescence.

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