Aeromicrobium panacisoli sp. nov. Isolated from Soil of Ginseng Cultivating Field

Abstract

A Gram-positive, rod-shaped, non-spore-forming, and aerobic bacterium (Gsoil 137^T) was isolated from soil of a ginseng field of Pocheon province in South Korea and subjected to a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 137^T was shown to belong to the family Nocardioidaceae and was closely related to Aeromicrobium ginsengisoli Gsoil 098^T (96.7%), Aeromicrobium panaciterrae (96.7%), and Aeromicrobium halocynthiae JCM 15749^T (96.6%). Being phylogenetic, it was most closely related to Aeromicrobium halocynthiae JCM 15749^T. The G+C content of the genomic DNA was 70.3 mol%. The diagnostic diamino acid of the cell wall peptidoglycan was LL-diaminopimelic acid. The predominant menaquinone was menaquinone MK-8 (H₄) and MK-7 (H4) was a minor compound. The major cellular fatty acids were C_14:0, C_16:0, C_18:1ω9c and summed feature 4 (C_16:1ω7c/C_15:0 iso 2-OH). All these data supported the affiliation of strain Gsoil 137^T to the genus Aeromicrobium. The results of physiological and biochemical tests enabled strain Gsoil 137^T to be differentiated genotypically and phenotypically from currently known Aeromicrobium species. Therefore, strain Gsoil 137^T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium panacisoli sp. nov. is proposed. The type strain is Gsoil 137^T (= KCTC 19130^T = DSM 17940^T = CCUG 52475^T).

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